What is the most important event in Mary's life that prepared her for a role as Theo-tokos? It’s her Immaculate Conception, yes, and that is being conceived without original sin. When Mary’s father, Saint Joaquim, and her mother, Saint Anne, produced her body by procreation, God directly created and infused Mary’s soul into the body at the precise moment of her conception in St. Anne’s womb, free from original sin.